LOCUS       40924_23874       12877 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB760 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12877)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12877)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12877)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12877)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1340..1370
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1424..2080
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2425..2727
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     misc_feature    2771..2895
                     /label=attR2
                     /note="attR2"
     protein_bind    complement(2771..2895)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2906..3616
                     /codon_start=1
                     /label=TagRFP
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     terminator      3641..3893
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3927..3943)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3951..3967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3975..4005)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4020..4041)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(4077..4329)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4367..5662)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61300.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(4367..5662)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5714..5893)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6399..6423)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6944..7732
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7981..8569
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8755..8895)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9239..9433)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9502..10572)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11004..11630)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcatggt gtctaagggc gaagagctga ttaaggagaa
     2941 catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca agtgcacatc
     3001 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg
     3061 cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcagaac
     3121 cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc ctgagggctt
     3181 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
     3241 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcccatc
     3301 caacggccct gtgatgcaga agaaaacact cggctgggag gccaacaccg agatgctgta
     3361 ccccgctgac ggcggcctgg aaggcagaag cgacatggcc ctgaagctcg tgggcggggg
     3421 ccacctgatc tgcaacttca agaccacata cagatccaag aaacccgcta agaacctcaa
     3481 gatgcccggc gtctactatg tggaccacag actggaaaga atcaaggagg ccgacaaaga
     3541 gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctagcaaact
     3601 ggggcacaaa cttaattgag cttagagctc gaatttcccc gatcgttcaa acatttggca
     3661 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     3721 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     3781 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     3841 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     3901 ggttccggaa ccaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     3961 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     4021 tgagtgagct aactcacatt aggctgaatt aggcgcgcct atttctatta attcccgatc
     4081 tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt
     4141 ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa
     4201 taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca gaaattatat
     4261 gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt
     4321 tgaacgatcg gggaaattcg cctcgagtga cttctctgat tgcagactat ttataccaac
     4381 cagcaagaaa gtgtcgaagt atgaagcaga aggcgcaggc tagagcctgg aaaacgagaa
     4441 gatggatgca aagagacttt tctgatttag gaccaaacaa cctcaagtaa acattgacga
     4501 gtgtcccatc ttttgtgccg gtcagtaatc cggtagcagg atcatacctt gggagacgat
     4561 ggcgcggaca tttcacaatc ccagcaagct gaggaagcga taatagaagg ttcagaactt
     4621 gaggaagaaa gaagatgagg agggtttcac tgaagtgacc cagaatacca acaactgcca
     4681 tagtcattcc ggcgaagact gtgtaagtgt ctccaacaaa aactgaagaa ggataccaat
     4741 tgtaagcaag cattgccaag gatgtggcca tcagcggttg agtaagaaat atcgagaagg
     4801 catgagcttg gtgatactcg ggatcaacag atgctccgat ttgcataacg ttgtgtatca
     4861 ggatagcagc ggcaataaca actgtttgac cgatttcaag gccattcaga ccagcgtgga
     4921 tgttaataga gtttgtacaa aagacagcaa gtagccccat atataactta tatatccttc
     4981 ctagattaag cacttccaag ccaatataag caacaagagg ttttggtata acaatagttg
     5041 tatgaccagc ataagccatc aaaagtggaa gggtagcaaa agatggcagg acaagtttta
     5101 ctctccaagg cacatcaagg acatcatcta caaatccaag caaaatcatg aagcaaatag
     5161 atgctagtgc tgcattatac tccacaagcc aattagaatc ttcagtgaag ttaaaatact
     5221 gaaatattat cgccacgatc aaaaagacaa taccaacaac aatacccaat gactctggca
     5281 ctttaatatc tccttgaggc gtgcctcttt tgttgatatc aaaaccaaac atgttacgtc
     5341 tcagaacgta tctggcagta acaggaatca ttttgagagt gacgaaaaac ccaaccaagc
     5401 taagccccgc gttaattaga atcgatcgct tgagttcatt atcaaccttg tagtgaaaac
     5461 agagaaggta tagatagagc gaacagagaa gagtagagat cacgaagatc actcctaatt
     5521 tcggcggcgc gagacgaaat tcctcaccgg aagcagaaac cgtctttcgg gtcatcttct
     5581 gctccgatgg cgctgagttg ggttccgacg ggtccggttt gttgggaatt gagatcgatg
     5641 aagctctctt acgagctgcc atatcttatt gccccccggg atcagatctg gattgagagt
     5701 gaatatgaga ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt
     5761 gacaagaaat atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg
     5821 tttctgacgt atgtgcttag ctcattaaac tccagaaacc cgcggctgag tggctccttc
     5881 aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg
     5941 tcataacgtg actcccttaa ttctccgctc atgatcttga tcccctgcgc catcagatcc
     6001 ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     6061 ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc
     6121 atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga
     6181 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
     6241 tgttccgctt cctttagcag cccttgcgcc ctgagtgctt gcggcagcgt gaagctgatc
     6301 cgtcgagatt aatagaaata aattcagcct aattcggcgt taattcagta cattaaaaac
     6361 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
     6421 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
     6481 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
     6541 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
     6601 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
     6661 aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     6721 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     6781 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     6841 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     6901 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg aagcggtgat
     6961 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     7021 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     7081 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     7141 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     7201 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     7261 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     7321 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     7381 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     7441 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     7501 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     7561 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     7621 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     7681 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
     7741 agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat
     7801 gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt
     7861 taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc
     7921 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7981 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8041 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8101 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     8161 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8221 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8281 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8341 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8401 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8461 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8521 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8581 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8641 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8701 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     8761 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     8821 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     8881 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     8941 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     9001 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     9061 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     9121 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     9181 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     9241 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     9301 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     9361 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     9421 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     9481 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     9541 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     9601 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     9661 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     9721 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     9781 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     9841 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     9901 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     9961 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
    10021 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
    10081 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
    10141 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
    10201 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
    10261 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
    10321 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
    10381 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
    10441 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
    10501 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
    10561 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
    10621 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
    10681 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
    10741 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
    10801 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
    10861 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
    10921 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
    10981 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
    11041 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
    11101 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
    11161 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
    11221 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
    11281 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
    11341 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
    11401 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
    11461 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
    11521 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
    11581 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
    11641 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
    11701 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
    11761 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
    11821 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
    11881 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
    11941 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
    12001 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
    12061 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
    12121 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
    12181 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
    12241 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
    12301 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
    12361 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
    12421 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
    12481 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
    12541 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
    12601 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
    12661 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
    12721 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
    12781 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
    12841 ttggcatgca catacaaatg gacgaacgga taaacct
//
